Three-Dimensional Hydrodynamic Focusing with a Two-Layer Microfluidic Device: Controlling the Vertical Position of the Focused Stream
نویسندگان
چکیده
A microfluidic manifold has been designed, fabricated, and tested which hydrodynamically focuses a sample into the center of a microchannel and provides control over the vertical position of the focused sample via the flow-rates of the focusing fluids [1]. To characterize the focusing action, a mixing experiment was performed in which the sample fluid and focusing fluid contained spectrally unique fluorescent dyes. By sweeping the ratio of the rate of the top focusing fluid to the rate of the bottom focusing fluid, the focused sample was positioned first near the top of the microchannel and then translated downward in steps to the bottom of the microchannel.
منابع مشابه
Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing.
In this work, we demonstrate an on-chip microfluidic flow cytometry system based on a three-dimensional (3D) hydrodynamic focusing technique, microfluidic drifting. By inducing Dean flow in a curved microfluidic channel, microfluidic drifting can be used to hydrodynamically focus cells or particles in the vertical direction and enables the 3D hydrodynamic focusing in a single-layer planar micro...
متن کامل"Microfluidic drifting"--implementing three-dimensional hydrodynamic focusing with a single-layer planar microfluidic device.
We introduce a novel fluid manipulation technique named "microfluidic drifting" to enable three-dimensional (3D) hydrodynamic focusing with a simple single-layer planar microfluidic device.
متن کاملSingle channel layer, single sheath-flow inlet microfluidic flow cytometer with three-dimensional hydrodynamic focusing.
Flow cytometry is a technique capable of optically characterizing biological particles in a high-throughput manner. In flow cytometry, three dimensional (3D) hydrodynamic focusing is critical for accurate and consistent measurements. Due to the advantages of microfluidic techniques, a number of microfluidic flow cytometers with 3D hydrodynamic focusing have been developed in recent decades. How...
متن کاملThree-dimensional Hydrodynamic Focusing over a Wide Reynolds Number Range Using a Two-layer Microfluidic Design
In this paper, we describe a microfluidic device with a novel two-layer microchannel structure to enable three-dimensional (3D) hydrodynamic focusing. Numerical results indicate that the device works reliably over a wide range of Reynolds numbers (Re). These results are confirmed using confocal laser scanning microscopy. The structure can be easily experimentally integrated into microfluidic sy...
متن کاملUniversally applicable three-dimensional hydrodynamic microfluidic flow focusing.
We have demonstrated a microfluidic device that can not only achieve three-dimensional flow focusing but also confine particles to the center stream along the channel. The device has a sample channel of smaller height and two sheath flow channels of greater height, merged into the downstream main channel where 3D focusing effects occur. We have demonstrated that both beads and cells in our devi...
متن کامل